beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 8H10D10]

SKU TA336770

Mouse Monoclonal beta-Actin Antibody (8H10D10)

Product Data

• Clone Name: 8H10D10
• Application: ELISA, FC, ICC/IF, Immunoblotting, WB
• Recommended Dilution: Flow (Intracellular): 1:200 - 1:400, Western Blot: 1:1000-1:5000, Simple Western: 12.5, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunoblotting
• Reactivity: Hamster, Human, Mouse, Primate, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
• Buffer: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 42 kDa
• Gene Name: actin, beta
• Database Link:
  NP_001092
  P60709
• Background: Beta Actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because Beta Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to Beta Actin provide a specific and useful tool in studying the intracellular distribution of Beta Actin and the static and dynamic aspects of the cytoskeleton.
• Synonyms: BRWS1; PS1TP5BP1
• Note: This Beta Actin (8H10D10) antibody is useful in Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence and ELISA.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis