Noxa (PMAIP1) Mouse Monoclonal Antibody [Clone ID: 114C307.1]

SKU TA336673

Mouse Monoclonal Noxa Antibody (114C307.1)

Product Data

• Clone Name: 114C307.1
• Application: FC, ICC/IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1 ug / million cells, Western Blot: 1-2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:20-1:1000, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: phorbol-12-myristate-13-acetate-induced protein 1
• Database Link:
  NP_066950
  Q13794
• Background: Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.
• Synonyms: APR; NOXA
• Note: Immunocytochemistry and Immunofluorescence; See Yakovlev et all (2003) for details.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: p53 signaling pathway