Neurofibromin (NF1) Rabbit Polyclonal Antibody

SKU TA336613

Rabbit Polyclonal Neurofibromin 1 Antibody

Product Data

• Application: ICC/IF, IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin: 1:200-1:500, Immunohistochemistry-Frozen: 1:200-1:500, Immunohistochemistry: 1:200-1:500, Western Blot: 1:500-1:2000
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50). [UniProt# P21359]
• Buffer: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: neurofibromin 1
• Database Link:
  NP_001121619
  P21359
• Background: Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).
• Synonyms: NFNS; VRNF; WSS
• Note: This Neurofibromin 1 antibody can be used for Western blot and Immunohistochemistry of frozen and paraffin-embedded sections. In WB, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23910086)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway