Actin (ACTA1) Mouse Monoclonal Antibody [Clone ID: mAbGEa]

SKU TA336491

Mouse Monoclonal Actin Antibody (mAbGEa)

Product Data

• Clone Name: mAbGEa
• Application: ELISA, FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Western Blot: 1:100-1:1000, ELISA: 1:100 - 1:2000, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Simple Western: 1:25, Immunocytochemistry/ Immunofluorescence: 1:20-1:100, Flow Cytometry: 1 ug/ml, Flow (Intracellular): 1 ug/ml
• Reactivity: Bovine, Drosophila, Hamster, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgM, kappa
• Clonality: Monoclonal
• Immunogen: Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46]
• Buffer: Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: IgM purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: actin, alpha 1, skeletal muscle
• Database Link:
  NP_001091
  P68133
• Background: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility.
• Synonyms: ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM
• Note: This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Stem cell - Pluripotency