CIKS (TRAF3IP2) Rabbit Polyclonal Antibody

SKU TA336472

Rabbit Polyclonal TRAF3IP2 Antibody

Product Data

• Application: IHC, IP, Simple Western, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1-2 ug/ml, Immunohistochemistry-Paraffin, Simple Western: 1:20, Immunoprecipitation
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was developed against a synthetic peptide (QDLPRPLRSREFPQFEP) corresponding to amino acids 225-241 of human TRAF3IP2.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: TRAF3 interacting protein 2
• Database Link:
  NP_679211
  O43734
• Background: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (3) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1/CIKS cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1/CIKS protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.
• Synonyms: ACT1; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; CIKS; PSORS13

Reference Data