D4 (ARHGDIB) Mouse Monoclonal Antibody [Clone ID: 97A1015]

SKU TA336465

Mouse Monoclonal D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015)

Product Data

• Clone Name: 97A1015
• Application: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 ug/10^6 cells, Immunohistochemistry: 1:10-1:500, Immunoprecipitation: 1-2 ug/ml, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin: 1:10-1:500, Flow (Intracellular): 0.5-1 ug/10^6 cells
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: Rho GDP dissociation inhibitor beta
• Database Link:
  NP_001166
  P52566
• Background: D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
• Synonyms: D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway