TLR4 Mouse Monoclonal Antibody [Clone ID: 76B357.1]
Specifications
| Product Data | |
| Clone Name | 76B357.1 |
|---|---|
| Application | Block/Neutralize, ChIP, CyTOF-ready, Dot, ELISA, FC, ICC/IF, IHC, SDS-PAGE, WB |
| Recommended Dilution | SDS-Page, Knockdown Validated, Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, ELISA, Block/Neutralize, Knockout Validated, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, In vitro assay, ELISA Capture (Matched Antibody Pair): 0.5 ug, Western Blot: 1-3 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry-Frozen: 1:500, CyTOF-ready, Dual RNAscope ISH-IHC |
| Reactivity | Human, Mouse, Rat, Squirrel |
| Antibody Host | Mouse |
| Isotype | IgG2b, kappa |
| Clonality | Monoclonal |
| Immunogen | This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564). |
| Buffer | PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration | lot specific |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | toll like receptor 4 |
| Database Link | |
| Background | The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6). |
| Synonyms | ARMD10; CD284; TLR-4; TOLL |
| Note | Flow (Intracellular) use reported in literature (See Cohen et al, 2008); Immunofluorescence (See Nowicki et al, 2009); IHC-F (Nowicki et al, 2012); Flow (cell surface) use reported in scientific literature (PMID 23796194) |
| Reference Data | |
| Protein Categories | CD Markers, Chemokines, Cytokines, Growth Factors, Intracellular Proteins, Membrane Proteins, Nervous system Diseases |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway |
Reviews
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| FAQs |
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