Caspase 1 (CASP1) Mouse Monoclonal Antibody [Clone ID: 14F468]

SKU TA336409

Mouse Monoclonal Caspase-1 Antibody (14F468)

Product Data

• Clone Name: 14F468
• Application: ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Frozen, Simple Western: 1:50, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin: 1:10-1:500, Immunohistochemistry: 1:100 - 1:500, Western Blot: 0.5-2 ug/ml
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 45 kDa
• Gene Name: caspase 1
• Database Link:
  NP_150634
  P29466
• Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
• Synonyms: ICE; IL1BC; P45
• Note: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway