HDAC2 Rabbit Polyclonal Antibody

CAT#: TA325112

Rabbit polyclonal HDAC2 Antibody (Center)

Product Images

HDAC2 Antibody (Center) (Cat. #TA325112) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow).

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  HDAC2 Antibody (Center) (Cat. #TA325112) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of Hela cell stained with HDAC2 Antibody (Center) (Cat#TA325112).Hela cells were incubated with HDAC2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). HDAC2 immunoreactivity is localized to Nucleus significantly.

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  HDAC2 Antibody (Center) (Cat. #TA325112) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, FC: 1:10~50, IF: 1:10~50
• Reactivities: Human (Predicted: Mouse, Chicken)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the Central region of human HDAC2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55364 Da
• Gene Name: histone deacetylase 2
• Database Link:
  NP_001518
  Entrez Gene 15182 MouseEntrez Gene 3066 Human
  UniProt ID Q92769
• Synonyms: HD2; RPD3; YAF1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer