CHMP5 Rabbit Polyclonal Antibody

CAT#: TA308272

Rabbit Polyclonal antibody to CHMP5 (chromatin modifying protein 5)

Product Images

Sample (30ug whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. 12% SDS PAGE. TA308272 diluted at 1:1000

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  Sample (50 ug of whole cell lysate). A: mouse stomach. 12% SDS PAGE. TA308272 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5 (TA308272) antibody at 1:100 dilution.

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  CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (TA308272) diluted at 1:500. Blue: Hoechst 33342 stain

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Pig, Bovine, Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 202 of CHMP5 (Uniprot ID#Q9NZZ3)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: charged multivesicular body protein 5
• Database Link:
  NP_057494
  Entrez Gene 76959 MouseEntrez Gene 51510 Human
  UniProt ID Q9NZZ3
• Background: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]). [supplied by OMIM]
• Synonyms: C9orf83; CGI-34; HSPC177; PNAS-2; SNF7DC2; Vps60
• Note: Seq homology of immunogen across species: Pig (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Pathways: Endocytosis