Bak (BAK1) Goat Polyclonal Antibody

CAT#: TA302647

Goat Polyclonal Antibody against BAK1

Product Images

TA302647 staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  Figure from citation: Western blot analysis of extracts from rat brains by using anti-Bak1 antibody showed the expression of Bak1 protein level was up-regulated in Rats were subjected to 2h of MCAO and 24h reperfusion after FNS. Dilution: 1:500 View Citation

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.5-1µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Pig)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence ASGQGPGPPRQE-C, from the N Terminus of the protein sequence according to NP_001179.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.2 kDa
• Gene Name: BCL2 antagonist/killer 1
• Database Link:
  NP_001179
  Entrez Gene 12018 MouseEntrez Gene 116502 RatEntrez Gene 578 Human
  UniProt ID Q16611
• Background: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq]
• Synonyms: BAK; BAK-LIKE; BCL2L7; CDN1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane