MAP1LC3A Rabbit Polyclonal Antibody

SKU TA301542

Rabbit Polyclonal Antibody against LC3

Product Data

• Application: ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation: 20 ug / 500 ug of lysate, Immunohistochemistry: 1:200-1:400, Simple Western: 1:50, Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence: 1:100-1:300, Immunohistochemistry-Paraffin: 1:200-1:400, Flow Cytometry, ELISA, Immunohistochemistry Whole-Mount, Immunoblotting, Immunohistochemistry-Frozen, Southern Blot, Western Blot: 2.0 ug/ml
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).
• Buffer: PBS, 0.02% Sodium Azide
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  NP_073729
  Q9H492
• Background: Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
• Synonyms: 1BLC3; ATG8F; LC3B; MAP1A; MAP1LC3B-a

Reference Data