Carbonic Anhydrase IX (CA9) Rabbit Polyclonal Antibody

SKU TA301431

Rabbit Polyclonal Antibody against Carbonic Anhydrase IX

Product Data

• Application: ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Western Blot: 1 - 3 ug/ml, Proximity Ligation Assay, Gel Super Shift Assays, Simple Western: 1:50, Immunoblotting, ELISA, Immunoprecipitation: 1:10 - 1:500, Microarray, Immunohistochemistry-Paraffin: 1:200 - 1:500, Immunocytochemistry/ Immunofluorescence: 2 - 5 ug/ml, Dual RNAscope ISH-IHC: 1:1000, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow Cytometry: 1:1000, Immunohistochemistry: 1:200 - 1:500, Immunohistochemistry-Frozen: 1:200 - 1:500
• Reactivity: Dog, Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from a C-terminal sequence of the human CA IX.
• Buffer: Tris-citrate/phosphate, pH 7-8, 0.1% sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: carbonic anhydrase 9
• Database Link:
  NP_001207
  Q16790
• Background: Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
• Synonyms: CAIX; MN

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism