Caspase 4 (CASP4) (N-term) Rabbit Polyclonal Antibody

SKU SP2145P

Caspase 4 (CASP4) (N-term) rabbit polyclonal antibody, Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1.0 µg/ml.; Immunohistochemistry on paraffin sections: 2.0 µg/ml. ; This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. ; This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.; Recommended Positive Control: Spleen tissue.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 16 amino acid peptide from Human Caspase-4 amino terminus
• Specificity: This antibody recognises an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. This antibody detects a band of approximately 34 kDa in Ramos cell lysates (predicted precursor MWT 31 kDa).
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: caspase 4
• Database Link:
  P49662
• Background: Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimers disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.
• Synonyms: CASP4, ICH2, ICE(rel)-II, Protease ICH-2, Protease TX

Reference Data