MIF Mouse Monoclonal Antibody [Clone ID: 4E4]

CAT#: SM6013

MIF mouse monoclonal antibody, clone 4E4, Purified

Product Images

Western blot analysis: The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human MIF antibody (1/1,000). Protein wasvisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast.

Specifications

Product Data

• Clone Name: 4E4
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/2,000. Recommended starting dilution is 1/1,000.
  Flow Cytometry.
  Immunofluorescence/Immnunocytochemistry.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human MIF (1-115 aa) purified from E. coli.
• Specificity: The antibody recognizes Human MIF.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Database Link:
  Entrez Gene 4282 Human
  UniProt ID P14174
• Background: The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
• Synonyms: Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF