MIF Mouse Monoclonal Antibody [Clone ID: 4E4]
Specifications
| Product Data | |
| Clone Name | 40000 |
|---|---|
| Application | ELISA, FC, ICC/IF, IF, IHC, WB |
| Recommended Dilution | ELISA. Western blot: 1/500-1/2,000. Recommended starting dilution is 1/1,000. Flow Cytometry. Immunofluorescence/Immnunocytochemistry. Immunohistochemistry on Paraffin Sections: 5 µg/ml. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant human MIF (1-115 aa) purified from E. coli |
| Specificity | The antibody recognizes Human MIF. Other species not tested. |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purification | By protein-G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Database Link | |
| Background | The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. |
| Synonyms | Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Reference Data | |
| Protein Categories | Allergies and autoimmune diseases, Chemokines, Cytokines, Intracellular Proteins, Secreated Proteins |
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