IRF5 (176-240) Mouse Monoclonal Antibody [Clone ID: 10T1]

CAT#: SM6008

IRF5 (176-240) mouse monoclonal antibody, clone 10T1, Purified

Product Images

Western blot analysis: The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 10T1
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/1,000.
  Immunoprecipitation.
  Immunohistochemistry on paraffin sections: 1/50.
  Immunocytochemistry / Immunoflourescence.
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human IRF-5 (176-240 aa) purified from E. coli
• Specificity: The antibody recognizes Interferon Regulatory Factor 5 (IRF-5) at aa 176-240.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon regulatory factor 5
• Database Link:
  Entrez Gene 3663 Human
  UniProt ID Q13568
• Background: IRF-5 is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.
• Synonyms: Interferon regulatory factor 5, IRF-5