GAPDHS Mouse Monoclonal Antibody [Clone ID: Hs-8]

SKU SM3115P

GAPDHS mouse monoclonal antibody, clone Hs-8, Purified

Product Data

• Clone Name: Hs-8
• Application: FC, IF, WB
• Recommended Dilution: Immunocytochemistry: 10 µg/ml, membrane permeabilization (acetone) essential.; Flow cytometry.; Western blot.
• Reactivity: Human, Porcine
• Antibody Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: The antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".
• Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0; State: Purified; State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Precipitation methods and size-exclusion chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Database Link:
  O14556
• Background: GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
• Synonyms: GAPDHS, GAPD2, GAPDS, HSD35, HSD-35

Reference Data