CD14 Mouse Monoclonal Antibody [Clone ID: MEM-18]

SKU SM3009AS

CD14 mouse monoclonal antibody, clone MEM-18, Azide Free

Product Data

• Clone Name: MEM-18
• Application: ELISA, FC, FN, IP, WB
• Recommended Dilution: Flow Cytometry: 4 µg/ml.; Immunoprecipitation.; Western blot (non-reducing conditions).; ELISA: This antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of Human CD14 in combination with clone B-A8.; Functional Assays: This antibody MEM-18 completely blocks binding of Fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
• Reactivity: Human, Primate
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.; In Human, the epitope recognized by MEM-18 is located between amino acids 57-64.
• Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized; State: Azide Free; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD14 molecule
• Database Link:
  P08571
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14

Reference Data