Fc epsilon RI (FCER1A) Mouse Monoclonal Antibody [Clone ID: 9E1]

CAT#: SM2251P

Fc epsilon RI (FCER1A) mouse monoclonal antibody, clone 9E1, Purified

Size: 100 ug 200 ug


USD 556.00

5 Days*

Size
    • 200 ug

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Specifications

Product Data
Clone Name 9E1
Applications ELISA, FC, IF, IHC, IP, WB
Recommended Dilution ELISA.
Western Blot.
Immunoprecipitation.
Immunofluorescence.
Flow Cytometry:
Use 10 µl of 1/25-1/50 diluted antibody to label 106 cells in 100 µl.
Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen CCR7 Rat basophilic leukaemia (RBL)-2H3 transfectants.
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Specificity SM2251P recognizes the high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response.
Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.
Formulation PBS, pH 7.4
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
Synonyms IgE Fc receptor subunit alpha, Fc-epsilon RI-alpha, FCE1A, FcERI
Reference Data

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