Fc epsilon RI (FCER1A) Mouse Monoclonal Antibody [Clone ID: 9E1]

SKU SM2251P

Fc epsilon RI (FCER1A) mouse monoclonal antibody, clone 9E1, Purified

Product Data

• Clone Name: 9E1
• Application: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA.; Western Blot.; Immunoprecipitation.; Immunofluorescence.; Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10⁶ cells in 100 µl.; Immunohistochemistry on Paraffin Sections: 10 µg/ml.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: CCR7 Rat basophilic leukaemia (RBL)-2H3 transfectants. ; Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
• Specificity: SM2251P recognizes the high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response.; Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.
• Buffer: PBS, pH 7.4; State: Purified; State: Liquid purified IgG fraction from Tissue Culture Supernatant; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: Fc fragment of IgE receptor Ia
• Database Link:
  P12319
• Background: Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
• Synonyms: IgE Fc receptor subunit alpha, Fc-epsilon RI-alpha, FCE1A, FcERI

Reference Data