CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

SKU SM2135LE

CD52 rat monoclonal antibody, clone YTH34.5, Low Endotoxin

Product Data

• Clone Name: YTH34.5
• Application: CT, ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA.; Western Blotting.; Immunoprecipitation.; Cytotoxic Assays: 50 µg/ml. Use human serum as complement source.; Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 1 x 10e6 cells in 100 µl.; Immunohistochemistry on Frozen Sections. ; Immunohistochemistry on Paraffin Sections. ; Immunohistochemistry on Resin Sections. ; This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (Ref.2).; Recommended Positive Control: Tonsil.
• Reactivity: Human, Monkey
• Antibody Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human lymphocytes.
• Specificity: SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1.; Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
• Buffer: PBS; State: Low Endotoxin; State: Liquid purified IgG fraction from tissue culture supernatant; Stabilizer: None; Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD52 molecule
• Database Link:
  P31358
• Background: The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Synonyms: HE5, CDw52

Reference Data