CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]

SKU SM2003

CD177 mouse monoclonal antibody, clone MEM-166, Purified

Product Data

• Clone Name: MEM-166
• Application: FC, IP, WB
• Recommended Dilution: Western blot (Non-reducing conditions).; Immunoprecipitation.; Flow Cytometry: Use 10µl of 1/50-1/100 diluted CD177 antibody to label 10⁶ cells in 100µl.
• Reactivity: Human, Monkey, Rhesus Monkey
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes CD177 (Neutrophil Glycoprotein NB1).; Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). ; MEM-166 recognizes CD177 under Non-Reducing Conditions.
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD177 molecule
• Database Link:
  Q8N6Q3
• Background: CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population.
• Synonyms: NB1, PRV1, HNA-2a, HNA2a

Reference Data