Anpep Rat Monoclonal Antibody [Clone ID: R3-63]

CAT#: SM1856P

Anpep rat monoclonal antibody, clone R3-63, Purified

Specifications

Product Data

• Clone Name: R3-63
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin sections: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
• Specificity: This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with Human CD13. Clone R3-63 has been reported to block Mouse APN enzyme activity. We recommend the use of SM1856A for this purpose.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: alanyl (membrane) aminopeptidase
• Database Link:
  Entrez Gene 16790 Mouse
  UniProt ID P97449
• Background: In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
• Synonyms: Aminopeptidase N, ANPEP, APN, PEPN