Anpep Rat Monoclonal Antibody [Clone ID: R3-63]
| Product Data | |
| Clone Name | R3-63 |
|---|---|
| Application | FC, IHC |
| Recommended Dilution | Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin sections: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details. |
| Reactivity | Mouse |
| Antibody Host | Rat |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line. |
| Specificity | This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with Human CD13. Clone R3-63 has been reported to block Mouse APN enzyme activity. We recommend the use of SM1856A for this purpose. |
| Buffer | PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | alanyl (membrane) aminopeptidase |
| Database Link | |
| Background | In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. |
| Synonyms | Aminopeptidase N, ANPEP, APN, PEPN |
| Reference Data | |
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