CD59 Rat Monoclonal Antibody [Clone ID: YTH53.1]

SKU SM1768T

CD59 rat monoclonal antibody, clone YTH53.1, Purified

Product Data

• Clone Name: YTH53.1
• Application: FC, IHC, WB
• Recommended Dilution: Western Blot.; Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.; Immunohistochemistry on Frozen Sections.; Immunohistochemistry on Paraffin Sections (1/5-1/10): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Positive Control Tissue: Placenta.
• Reactivity: Human
• Antibody Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Peripheral Blood T cells.; Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.
• Specificity: This antibody recognises the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes.; Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays.; CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
• Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.; State: Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD59 molecule
• Database Link:
  P13987
• Background: CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
• Synonyms: MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20

Reference Data