C5R1 (C5AR1) Mouse Monoclonal Antibody [Clone ID: P12/1]

SKU SM1666P

C5R1 (C5AR1) mouse monoclonal antibody, clone P12/1, Purified

Product Data

• Clone Name: P12/1
• Application: FC, IHC, WB
• Recommended Dilution: Western blot.; Immunohistochemistry on Frozen Sections.; Immunohistochemistry on Paraffin Sections.; Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 5 x 10e5 cells in 100 µl.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C5aR - peptide: Met1 - Asn31.; Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
• Specificity: This antibody recognises the C5a receptor (C5aR) CD88.; Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31).; Clone P12/1 does not inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
• Buffer: PBS containing 0.09% Sodium Azide; State: Purified; State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: complement component 5a receptor 1
• Database Link:
  P21730
• Background: C5a Anaphylatoxin Receptor mediates the effects of one of the most potent chemotactic agents known, in a wide variety of inflammatory cell types. C5a receptor 1 has been implicated in asthma. CD88 is predominantly expressed on cells of the myeloid lineage.
• Synonyms: C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1

Reference Data