CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

CAT#: SM1526R

CD81 / TAPA1 hamster monoclonal antibody, clone Eat2, PE

Specifications

Product Data

• Clone Name: Eat2
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: 38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
• Specificity: This antibody recognises and CD81. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD81 antigen
• Database Link:
  Entrez Gene 12520 Mouse
  UniProt ID P35762
• Background: CD81 is a 26kD cell surface glycoprotein that is also known as TAPA-1.In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
• Synonyms: S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28