CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]
Specifications
| Product Data | |
| Clone Name | Eat2 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
| Reactivity | Mouse |
| Antibody Host | Hamster |
| Isotype | IgG |
| Clonality | Monoclonal |
| Immunogen | 38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line. |
| Specificity | This antibody recognises and CD81. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding. |
| Buffer | PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE) |
| Reconstitution Method | Restore with 1.0 ml distilled water. |
| Purification | Affinity Chromatography on Protein G. |
| Conjugation | PE |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD81 antigen |
| Database Link | |
| Background | CD81 is a 26kD cell surface glycoprotein that is also known as TAPA-1.In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. |
| Synonyms | S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28 |
| Reference Data | |
| Protein Categories | CD Markers, Cytokines, Immune system diseases, Membrane Proteins |
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