CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

SKU SM1150P

CD62P (SELP) mouse monoclonal antibody, clone AK-6, Purified

Product Data

• Clone Name: AK-6
• Application: FC, IHC, WB
• Recommended Dilution: Western blotting.; Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 10⁶ cells in 100 µl.; Immunohistochemistry on Frozen Sections: 1/1000-1/5000.; Immunohistochemistry on Paraffin Embedded Sections: 1/500-1/2000. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0.; Recommended Positive Control Tissue: Tonsil.
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human platelet membrane glycoproteins.
• Specificity: This antibody recognizes CD62P.; It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.
• Buffer: PBS, pH 7.4; State: Purified; State: Liquid purified IgG fraction from Tissue Culture Supernatant; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: selectin P
• Database Link:
  P16109
• Background: CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3

Reference Data