Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

SKU R1550BS

Alanine aminotransferase 1 / ALT1 sheep polyclonal antibody, Biotin, Purified

Product Data

• Application: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3,000.; ELISA: 1/5,000-1/25,000.; This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
• Reactivity: Porcine
• Antibody Host: Sheep
• Clonality: Polyclonal
• Immunogen: Alanine transaminase (ALT) from pig heart
• Specificity: This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
• Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2; Label: Biotin; State: Purified; State: Lyophilized purified Ig fraction; Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free); Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P13191
• Background: Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
• Synonyms: GPT, AAT1, GPT1

Reference Data