Cyanine Dyes Sheep Polyclonal Antibody

SKU R1460B

Cyanine Dyes sheep polyclonal antibody, Biotin

Product Data

• Application: ELISA, FC, IF, IHC
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.; Recommended dilutions: This product has been assayed against 1.0 ug of Cyanine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.; A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
• Antibody Host: Sheep
• Clonality: Polyclonal
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum and cyanine conjugated IgG.; Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% Sodium Azide as preservative and 10 mg/ml BSA as stabilzer, IgG and Protease free.; Label: Biotin; State: Lyophilized purified Ig fraction; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecule per sheep IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient

Reference Data