pgmB Goat Polyclonal Antibody

SKU R1157PS

pgmB goat polyclonal antibody, Purified

Product Data

• Application: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3000.; ELISA: 1/1,000-1/5,000 dilution suggested to begin optimization.; This product has been assayed against 1.0 µg of beta-phosphoglucomutase [Lactococcus lacti] in a standard Sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG [H&L] (Rabbit) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested.
• Reactivity: Lactococcus lacti
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Beta-phosphoglucomutase (PGMB) from Lactococcus lacti
• Specificity: This antibody detects beta-phosphoglucomutase from Lactococcus lacti.; Immunoelectrophoresis gives a single precipitin arc against anti-Goat serum as well as purified and partially purified beta-phosphoglucomutase [Lactococcus lacti]. ; Cross reactivity against b-Phosphoglucomutase from other sources is unknown.
• Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2; State: Purified; State: Lyophilized purified Ig fraction; Stabilizer: None; Preservative: 0.01% Sodium Azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multistep process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P71447
• Synonyms: Beta-phosphoglucomutase, Beta-PGM, Phosphoglucomutase beta, Glucose Phosphomutase
• Note: Conjugates available. Please ask for details.

Reference Data