FGF2 Rabbit Polyclonal Antibody

SKU DP3506S

FGF2 rabbit polyclonal antibody, Azide Free

Product Data

• Application: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human FGF-2 basic; (5 ng/ml) on HUVEC cells, a concentration of about 3.5 µg/ml of this antibody is required.; Western Blot: 2-5 µg/ml.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) recombinant Human FGF-2 (basic) [Ala136 – Ser288] produced in E.coli (Cat.-No DA3503XC).
• Specificity: The antibody will detect Human FGF-2 (basic).
• Buffer: PBS, pH 7.4 without stabilizer or preservative; State: Azide Free; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: fibroblast growth factor 2
• Database Link:
  P09038
• Background: FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Synonyms: FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2

Reference Data