CD66a/b/c/e Mouse Monoclonal Antibody [Clone ID: TET2]

CAT#: DM1205

CD66a/b/c/e mouse monoclonal antibody, clone TET2, Purified

Product Images

Figure 1. FACS analysis of BOSC23 cells using TET2 /DM1205. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM1, 5, 6, 8 (Red curves) or an irrelevant protein (Control transfectant). Binding ofTET2 was detected with a PE

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  Figure 2. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected wi

Specifications

Product Data

• Clone Name: TET2
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1.0 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200.
  Western Blot: 4 µg/ml.
  Immunohistology: 1-2 µg/10⁶ cells (on Cryosections).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: TET2 was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
• Specificity: This antibody recognizes CD66a/b/c/e.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immuno-globulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
• Synonyms: CEACAM1, CEACAM5, CEACAM6, CEACAM8, BGP, CD66a, CD66b, CD66c, CD66d
• Note: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.