MHC Class I (RT1Ac) Mouse Monoclonal Antibody [Clone ID: OX-27]

SKU CL129R

MHC Class I (RT1Ac) mouse monoclonal antibody, clone OX-27, PE

Product Data

• Clone Name: OX-27
• Application: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 10e6 cells in 100 µl.
• Reactivity: Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: PHA activated rat lymphocytes
• Specificity: This antibody recognises a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
• Buffer: PBS containing 1% BSA and 0.09% sodium azide; Label: PE; State: Lyophilized purified IgG fraction; Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water.
• Purification: Protein G chromatography
• Conjugation: PE
• Storage: Prior to and following reconstitution store (undiluted) at 2-8°C. DO NOT FREEZE.; This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Database Link:
  Q31255
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
• Synonyms: RT1-A3

Reference Data