MSH2 Mouse Monoclonal Antibody [Clone ID: OTI10F7]

CAT#: CF814210

Carrier-free (BSA/glycerol-free) MSH2 mouse monoclonal antibody, clone OTI10F7

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-MSH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814210)

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Specifications

Product Data

• Clone Name: OTI10F7
• Applications: IHC
• Recommended Dilution: IHC 1:30
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-304 of human MSH2 (NP_000242) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104.7 kDa
• Gene Name: mutS homolog 2
• Database Link:
  NP_000242
  Entrez Gene 17685 MouseEntrez Gene 81709 RatEntrez Gene 4436 Human
  UniProt ID P43246
• Background: This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
• Synonyms: COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Mismatch repair, Pathways in cancer