CD133 (PROM1) Mouse Monoclonal Antibody [Clone ID: OTI4E3]

SKU CF813577

Carrier-free (BSA/glycerol-free) PROM1 mouse monoclonal antibody, clone OTI4E3

Product Data

• Clone Name: OTI4E3
• Application: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PROM1 (NP_006008) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 97 kDa
• Gene Name: prominin 1
• Database Link:
  NP_006008
  O43490
• Background: This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
• Synonyms: AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane