Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI3H6]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT3 (<a href="https://www.origene.com/catalog/cdna-clones/expression-plasmids/rc215771-septin-3-sept3-nm-145733-human-tagged-orf-clone" title="RC215771" class="">RC215771</a>, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT3 (1:2000). Positive lysates <a href="https://www.origene.com/catalog/proteins/over-expression-lysates/ly403438-septin-3-sept3-nm-145733-human-over-expression-lysate" title="LY403438" class="">LY403438</a> (100ug) and <a href="https://www.origene.com/catalog/proteins/over-expression-lysates/lc403438-septin-3-sept3-nm-145733-human-over-expression-lysate" title="LC403438" class="">LC403438</a> (20ug) can be purchased separately from OriGene.

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Antibodies made against full-length proteins as antigens.

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SKU

CF811196

Carrier-free (BSA/glycerol-free) SEPT3 mouse monoclonal antibody,clone OTI3H6

Datasheet SDS

$620.00

3 Days*

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Specifications

Specifications
Product Data
Clone Name	OTI3H6
Application	IHC, WB
Recommended Dilution	WB 1:500~2000, IHC 1:500
Reactivity	Human, Mouse
Antibody Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Full length human recombiant protein of human SEPT3 (NP_663786) produced in HEK293T cell.
Buffer	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Shipping	Ambient
Predicted Protein Size	40.5 kDa
Gene Name	septin 3
Database Link	NP_663786 Q9UH03
Synonyms	bK250D10.3; SEP3
Reference Data
Protein Categories	Cytokines, Intracellular Proteins

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for CF811196

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Other "OTI3H6" antibodies (4)

SKU	Description	Size	Price
TA811196	SEPT3 mouse monoclonal antibody,clone OTI3H6	100 ul	$447.00
TA811196AM	SEPT3 mouse monoclonal antibody,clone OTI3H6, Biotinylated	100 ul	$509.00
TA811196BM	SEPT3 mouse monoclonal antibody,clone OTI3H6, HRP conjugated	100 ul	$509.00
TA811196S	SEPT3 mouse monoclonal antibody,clone OTI3H6	30 ul	$200.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI3H6]

View other "Septin 3 or NM_145733" antibodies

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Citations