GTP cyclohydrolase 1 (GCH1) Mouse Monoclonal Antibody [Clone ID: OTI5A1]

CAT#: CF810265

Carrier-free (BSA/glycerol-free) GCH1 mouse monoclonal antibody,clone OTI5A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCH1 (Cat# RC224968, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCH1 (Cat# TA810265)(1:2000). Positive lysates LY422611 (100ug) and LC422611 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human skin tissue within the normal limits using anti-GCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810265) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-GCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810265) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen carcinoma tissue within the normal limits using anti-GCH1 mouse monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GCH1 (NP_001019195) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.7 kDa
• Gene Name: GTP cyclohydrolase 1
• Database Link:
  NP_001019195
  Entrez Gene 14528 MouseEntrez Gene 29244 RatEntrez Gene 2643 Human
  UniProt ID P30793
• Background: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008]
• Synonyms: DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways