Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: OTI3B9]

SKU CF809934

Carrier-free (BSA/glycerol-free) TOP1 mouse monoclonal antibody,clone OTI3B9

Product Data

• Clone Name: OTI3B9
• Application: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 93-151 of human TOP1(NP_003277 ) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 90.5 kDa
• Gene Name: DNA topoisomerase I
• Database Link:
  NP_003277
  P11387
• Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
• Synonyms: TOPI

Reference Data

• Protein Families: Druggable Genome