IQGAP1 Mouse Monoclonal Antibody [Clone ID: OTI7F5]

SKU CF809928

Carrier-free (BSA/glycerol-free) IQGAP1 mouse monoclonal antibody,clone OTI7F5

Product Data

• Clone Name: OTI7F5
• Application: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:5000
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 464-609 of human IQGAP1(NP_003861) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 189.1 kDa
• Gene Name: IQ motif containing GTPase activating protein 1
• Database Link:
  NP_003861
  P46940
• Background: This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]
• Synonyms: HUMORFA01; p195; SAR1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Regulation of actin cytoskeleton