Semaphorin 3D (SEMA3D) Mouse Monoclonal Antibody [Clone ID: OTI2H4]

SKU CF809917

Carrier-free (BSA/glycerol-free) SEMA3D mouse monoclonal antibody,clone OTI2H4

Product Data

• Clone Name: OTI2H4
• Application: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 200-291 of human SEMA3D(NP_689967) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 89.5 kDa
• Gene Name: semaphorin 3D
• Database Link:
  NP_689967
  O95025
• Background: Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin. [UniProtKB/Swiss-Prot Function]
• Synonyms: coll-2; Sema-Z2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Axon guidance