SPI1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

SKU CF808850

Carrier-free (BSA/glycerol-free) SPI1 mouse monoclonal antibody,clone OTI1F3

Product Data

• Clone Name: OTI1F3
• Application: WB
• Recommended Dilution: WB 1:2000
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPI1(NP_003111) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 30.9 kDa
• Gene Name: Spi-1 proto-oncogene
• Database Link:
  NP_003111
  P17947
• Background: This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: OF; PU.1; SFPI1; SPI-1; SPI-A

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer