BCL2 Mouse Monoclonal Antibody [Clone ID: OTI9D3]

CAT#: CF806591

Carrier-free (glycerol/BSA-free) BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BCL2 (Cat# RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2 (Cat# TA806591). Positive lysates LY424602 (100ug) and LC424602 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-BCL2 monoclonal antibody (1:200).

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-BCL2 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and BCL2-Knockout HeLa cells (KO, Cat# LC811172) were separated by SDS-PAGE and immunoblotted with anti-BCL2 monoclonal antibody TA806591, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806591)

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Specifications

Product Data

• Clone Name: OTI9D3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCL2 (NP_000624) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: BCL2 apoptosis regulator
• Database Link:
  NP_000624
  Entrez Gene 12043 MouseEntrez Gene 24224 RatEntrez Gene 596 Human
  UniProt ID P10415
• Synonyms: Bcl-2; PPP1R50

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer