DOT1L Mouse Monoclonal Antibody [Clone ID: OTI6E7]

SKU CF802738

Carrier-free (BSA/glycerol-free) DOT1L mouse monoclonal antibody, clone OTI6E7 (formerly 6E7)

Product Data

• Clone Name: OTI6E7
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 164.7 kDa
• Gene Name: DOT1 like histone lysine methyltransferase
• Database Link:
  NP_115871
  Q8TEK3
• Background: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
• Synonyms: DOT1; KMT4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation