GAPDH Mouse Monoclonal Antibody [Clone ID: OTI2F8]

CAT#: CF802563

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Carrier-free (BSA/glycerol-free) GAPDH mouse monoclonal antibody, clone OTI2F8 (formerly 2F8)



  View other "OTI2F8" antibodies (4)

USD 600.00


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Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI2F8
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 35.9 kDa
Gene Name Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA.
Background The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Synonyms G3PD; GAPD; HEL-S-162eP
Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

Other Versions

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