HIF 2 alpha (EPAS1) Mouse Monoclonal Antibody [Clone ID: OTI2A6]

SKU CF801749

Carrier-free (BSA/glycerol-free) EPAS1 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)

Product Data

• Clone Name: OTI2A6
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 584-870 of human EPAS1 (NP_001421) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 96.3 kDa
• Gene Name: endothelial PAS domain protein 1
• Database Link:
  NP_001421
  Q99814
• Background: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
• Synonyms: bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma