TLE 1 (TLE1) Mouse Monoclonal Antibody [Clone ID: OTI1F5]

SKU CF800294

Carrier-free (BSA/glycerol-free) TLE1 mouse monoclonal antibody, clone OTI1F5 (formerly 1F5)

Product Data

• Clone Name: OTI1F5
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 83 kDa
• Gene Name: TLE family member 1, transcriptional corepressor
• Database Link:
  NP_005068
  Q04724
• Synonyms: ESG; ESG1; GRG1

Reference Data

• Protein Families: Transcription Factors