CD1C Mouse Monoclonal Antibody [Clone ID: OTI2F4]

CAT#: CF505411

Carrier-free (BSA/glycerol-free) CD1C mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell lines by using anti-CD1C monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD1C mouse monoclonal antibody. (TA505411)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CD1C mouse monoclonal antibody. (TA505411)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD1C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505411)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD1C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505411)

• Anti-CD1C mouse monoclonal antibody (TA505411) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD1C (RC218490).

• Immunofluorescent staining of HeLa cells using anti-CD1C mouse monoclonal antibody (TA505411).

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Specifications

Product Data

• Clone Name: OTI2F4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD1C(NP_001756) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens CD1c molecule (CD1C), mRNA.
• Database Link:
  NP_001756
  Entrez Gene 911 Human
  UniProt ID P29017
• Background: This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul
• Synonyms: BDCA1; CD1; CD1A; R7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage